European Light Microscopy Initiative

European Light Microscopy Initiative (ELMI), established in 2001, is a communication network between European researchers working in the field of light microscopy and manufacturers of such equipment. ELMI provides an up-to-date list of organized meetings and conferences, courses and workshops, and jobs within the field of light microscopy (imaging/microscopy specialists, core facility managers, image analysts, post-doc positions, etc.).

European Light Microscopy Initiative

Light Microscopy Resources

Light (fluorescence) microscopy resources detailing fundamental concepts in microscopy, structure and function of components of modern microscopes, modern microscopy systems such as laser scanning and spinning disk confocal systems, and plethora of advanced microscopy methods such as multi-photon microscopy, super-resolution imaging, deconvolution, and spectral unmixing.

Zeiss Campus

Nikon MicroscopyU

Leica Science Lab

Olympus Microscopy Resource Center

Molecular Expressions Optical Microscopy Primer

Electron Microscopy Resources

Electron microscopy resources detailing fundamental electron microscopy methods including transmission electron microscopy (TEM) and scanning electron microscopy (SEM), various methods of sample preparation, and applications in the field of bioscience and biomedicine.

ThermoFisher Scientific Electron Microscopy Resource

MyScope Electron and Light Microscopy Resource

Fluorescent Spectraviewers

Spectraviewers where excitation and emission maxima and spectra of a single fluorescent molecule (fluorescent protein/dye) or a combination of two or more fluorescent molecules can be viewed. Spectraviewers are essential to determine excitation and emission parameters of a fluorescent molecule, establish the level of bleed-through in experiments with two or more fluorescent labels, and optimize imaging experiments using virtual laser lines or lamp sources and excitation and emission filter sets.

ThermoFisher Scientific Spectraviewer

BioLegend Spectraviewer

BD Biosciences Spectraviewer

Chroma Spectraviewer

FPbase Spectraviewer

Expedeon Spectraviewer

Fluorescent Protein Properties

Interactive resources displaying various properties (excitation and emission wavelengths, extinction coefficient, quantum yield, brightness, stability, maturation, etc.) of fluorescent proteins including photoactivatable, photoconvertible, and photoswitchable fluorescent proteins.

Interactive Visualization of Fluorescent Protein Properties

Interactive Visualization of Photoswitchable Fluorescent Protein Properties

Cell Staining Tool

Cell staining tool that allows in-depth planning of imaging experiments in live and fixed cells. Large number of structures including but not limited to nucleus, mitochondria, and cytoskeleton can be stained with fluorescent labels (fluorescent proteins and dyes) of different colors allowing live and fixed cell experiments using one, two, or more fluorescent labels within one cell.

ThermoFisher Scientific Cell Staining Tool