Turku Cell Imaging and Cytometry Core Facility has teamed up with the Oxford-Zeiss Centre of Excellence at the Kennedy Institute of Rheumatology at the University of Oxford to organize a joint “Practical Course on Deep Tissue Imaging”. This course will take place in Oxford, UK on November 6-10.
Course Description:
Deep imaging (>50 μm) in biomedical samples such as thick tissue sections, spheroids, and organoids with fluorescence microscopy has become a much more common quest by bio-medical researchers. Yet, such imaging is very challenging as a consequence of difficulties with deep penetrative staining, and with optical limitations including light penetration, light scattering, and refractive index mismatch. The aim of this course is to collectively explore the use of a range of state-of-the-art fluorescence microscopes that are available within the Oxford – ZEISS Centre of Excellence at the University of Oxford, in order to gain a better understanding of the advantages and limitations of these microscopes for imaging of thicker samples. This course will include lectures, practical demonstrations, and hands on use with designated samples (1. 100 um thick tissue sections, and 2. Spheroids) on five different light microscopy imaging modalities:
- Standard widefield microscopy,
- Laser-scanning confocal microscopy,
- Multi-photon microscopy,
- Lattice light-sheet microscopy,
- Lattice structured illumination microscopy.
The course will furthermore include lectures and practical demonstrations of image analysis tools that are applicable for analysing images acquired within the course. At the end of the course, attendees will have gained a theoretical background and practical understanding of the various microscope modalities above. In addition, attendees will have gained invaluable practical experience with optimisation of imaging deep into tissue and spheroids.
Venue: University of Oxford, UK
Dates: November 6-10, 2023
Course Organizers:
Kseniya Korobchevskaya, Helena Coker & Marco Fritzsche, Kennedy Institute of Rheumatology (KIR), University of Oxford, UK.
Christoffer Lagerholm, Cell Imaging and Cytometry Core, Turku Bioscience Centre, Åbo Akademi University, Finland.
James Bancroft & Edward Drydale, Wellcome Trust Centre for Human Genetics, University of Oxford, UK.
The organisers would like to thank ZEISS for their support.
For more information, contact reception@kennedy.ox.ac.uk
